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Catalog Number | orb1262503 |
---|---|
Category | Antibodies |
Description | SCNN1A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | FC, IF, IHC-P, WB |
Reactivity | Human |
Isotype | Rabbit Ig |
Immunogen | This SCNN1A antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 365-391 amino acids from the Central region of human SCNN1A. |
Concentration | batch dependent |
Dilution range | For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 76 kDa |
Target | SCNN1A |
UniProt ID | P37088 |
NCBI | P37088 |
Storage | Store at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
Buffer/Preservatives | Supplied in PBS with 0.09% (W/V) sodium azide. |
Alternative names | Amiloride-sensitive sodium channel subunit alpha, Read more... |
Note | For research use only |
Application notes | For WB starting dilution is: 1:2000For FACS starting dilution is: 1:25For IHC-P starting dilution is: 1:10~50For IF starting dilution is: 1:10~50 |
Expiration Date | 12 months from date of receipt. |
Western Blot at 1:2000 dilution + Daudi whole cell lysate Lysates/proteins at 20 ug per lane.
Overlay histogram showing WiDr cells stained with Antibody (green line). The cells were fixed with 2% paraformaldehyde (10 min). The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (1:25 dilution) for 60 min at 37°C. The secondary antibody used was Goat-Anti-Rabbit IgG, Conjugated Highly Cross-Adsorbed at 1/200 dilution for 40 min at 37°C. Isotype control antibody (blue line) was rabbit IgG (1ug/1x10^6 cells) used under the same conditions. Acquisition of > 10000 events was performed.
Formalin-fixed and paraffin-embedded human lung carcinoma reacted with SCNN1A Antibody, which was peroxidase-conjugated to the secondary antibody, followed by DAB staining.
Confocal immunofluorescent analysis of SCNN1A Antibody with Hela cell followed by Alexa Fluor489-conjugated goat anti-rabbit lgG (green). DAPI was used to stain the cell nuclear (blue).
Flow cytometric analysis of WiDr cells (right histogram) compared to a negative control cell (left histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
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