You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1257449 |
---|---|
Category | Antibodies |
Description | SCNN1A Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 105-225 of human SCNN1A (NP_001029.1). |
Concentration | batch dependent |
Dilution range | WB: 1:200 - 1:2000IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 76kDa |
Target | SCNN1A |
UniProt ID | P37088 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Amiloride-sensitive sodium channel subunit alpha, Read more... |
Note | For research use only |
Application notes | WB: 1:200 - 1:2000IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SCNN1A antibody (orb1257449) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of human lung cancer cells using SCNN1A antibody (orb1257449) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (orb1257449) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of mouse kidney cells using SCNN1A antibody (orb1257449) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of rat lung cells using SCNN1A antibody (orb1257449) at dilution of 1:100. Blue: DAPI for nuclear staining.
ICC, IF, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
ICC, IF, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating