You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1257020 |
---|---|
Category | Antibodies |
Description | SCG2 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, WB |
Reactivity | Human |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 30-320 of human SCG2 (NP_003460.2). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IF: 1:10 - 1:100 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 65kDa |
Target | SCG2 |
UniProt ID | P13521 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | SCG2, secretogranin II, CHGC, EM66, SN, SgII, chro Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IF: 1:10 - 1:100 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using SCG2 antibody (orb1257020) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 90s.
Immunofluorescence analysis of U2OS cells using SCG2 antibody (orb1257020). Blue: DAPI for nuclear staining.
ICC, IF, IHC-P | |
Guinea pig, Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
Filter by Rating