SCF (KITLG) Antibody (C-term)

• Catalog Number: orb1935430
• Category: Antibodies
• Description: Purified Rabbit Polyclonal Antibody (Pab)
• Species/Host: Rabbit
• Clonality: Polyclonal
• Clone Number: RB13450
• Tested applications: FC, IF, WB
• Predicted Reactivity: Equine, Porcine
• Reactivity: Human
• Isotype: Rabbit IgG
• Dilution range: IF: 1:10~50, WB: 1:1000, WB: 1:1000, FC: 1:10~50
• Form/Appearance: Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS.
• Conjugation: Unconjugated
• MW: 30899 Da
• Target: This SCF (KITLG) antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 244-273 amino acids from the C-terminal region of human SCF (KITLG).
• UniProt ID: P21583
• NCBI: NP_000890.1, NP_003985.2
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles
• Alternative names: Kit ligand, Mast cell growth factor, MGF, Stem cel
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of KITLG Antibody (C-term) in 293 cell line lysates (35 ug/lane). KITLG (arrow) was detected using the purified Pab.

Western blot analysis of KITLG (arrow) using rabbit polyclonal KITLG Antibody (C-term). 293 cell lysates (2 ug/lane) either nontransfected (Lane 1) or transiently transfected with the KITLG gene (Lane 2).

Flow cytometric analysis of WiDr cells using SCF (KITLG) Antibody (C-term) (bottom histogram) compared to a negative control cell (top histogram). FITC-conjugated goat-anti-rabbit secondary antibodies were used for the analysis.

Immunofluorescence analysis of anti-KITLG Antibody (C-term) in HeLa cells. 0.025 mg/ml primary antibody was followed by Alexa-Fluor-546-conjugated donkey anti-rabbit lgG (H+L). Alexa-Fluor-546 emits orange fluorescence. Blue counterstaining is DAPI.