SCARB2 antibody

• Catalog Number: orb390190
• Category: Antibodies
• Description: Rabbit polyclonal antibody to SCARB2.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, WB
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: Recombinant fusion protein containing a sequence corresponding to amino acids 200-380 of human SR-B2/LIMPII (NP_005497.1).
• Dilution range: WB: 1:1000-3000
• Conjugation: Unconjugated
• MW: 80kDa
• UniProt ID: Q14108
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: PBS with 0.01% thimerosal,50% glycerol,pH7.3.
• Alternative names: SCARB2, AMRF, CD36L2, EPM4, HLGP85, LGP85, LIMP-2,
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunofluorescence analysis of L929 cells using SR-B2/LIMPII Rabbit pAb (orb390190) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Immunofluorescence analysis of U20S cells using SR-B2/LIMPII Rabbit pAb (orb390190) at dilution of 1:100 (40x lens). Secondary antibody: Cy3 Goat Anti-Rabbit IgG (H+L) at 1:500 dilution. Blue: DAPI for nuclear staining.

Western blot analysis of various lysates using SR-B2/LIMPII Rabbit pAb (orb390190) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 µg per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.