SATB2 antibody

• Catalog Number: orb2276447
• Category: Antibodies
• Description: SATB2 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Rabbit
• Clonality: Monoclonal
• Clone Number: Biorbyt-702R
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG
• Immunogen: Synthetic peptide corresponding to residues within aa200-300 corresponding to human SATB2
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: Q9UPW6
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: DNA-binding protein SATB2; GLSS; SATB homeobox 2;
• Note: For research use only
• Application notes: Positive Control: Colon: A strong nuclear staining should be seen in virtually all columnar epithelial cells. Negative Control: Colon: SATB2 staining should be absent in stromal and smooth muscle cells. Cellular Localization: Nuclear Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.

Colon Colorectal adenocarcinoma showing strong SATB2 immunostaining of all tumor cells.

A strong nuclear SATB2 immunostaining is seen in all epithelial cells of the normal colorectal mucosa.