SART1 Antibody

• Catalog Number: orb1246983
• Category: Antibodies
• Description: SART1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Predicted Reactivity: Bovine, Mouse, Rat
• Reactivity: Human
• Immunogen: The immunogen for this antibody is: GSSKKHRGEKEAA-C
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: SART1
• UniProt ID: O43290
• NCBI: NP_005137.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: SART1, squamous cell carcinoma antigen recognised
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:64000.Western Blot:Approx 125kDa band observed in HeLa lysates (consistent with Vertegaal et al, below, calculated MW of 90.3 kDa according to NP_005137). Recommended concentration: 0.03-0.1ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1246983 (0.03 ug/ml) staining of Hela lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.