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    SARS-CoV S Recombinant Protein (R667A, K968P, V969P)

    Catalog Number: orb1231536

    DispatchUsually dispatched within 5-10 working days
    $ 1,183.00
    Catalog Numberorb1231536
    CategoryProteins
    DescriptionSARS-CoV S Recombinant Protein (R667A, K968P, V969P)
    Tested applicationsELISA, WB
    TagHis Tag
    Dilution rangeThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Form/AppearanceLyophilized
    Purity> 95% as determined by SDS-PAGE.
    MW136.3 kDa
    Protein SequenceSer 14 - Pro 1195
    SourceHEK293 cells
    NCBIK968, R667, V969, AAP13567.1
    StorageLyophilized Protein should be stored at -20°C or lower for long term storage. Upon reconstitution, working aliquots should be stored at -20°C or -70°C. Avoid repeated freeze-thaw cycles.
    Buffer/PreservativesPBS
    Alternative namesSpike,S protein,Spike glycoprotein,S glycoprotein
    Read more...
    NoteFor research use only
    Application notesThis protein carries a polyhistidine tag at the C-terminus. The protein has a calculated MW of 136.3 kDa. The protein migrates as 190-210 kDa under reducing (R) condition (SDS-PAGE) due to glycosylation.
    Expiration Date6 months from date of receipt.
    SARS-CoV S Recombinant Protein (R667A, K968P, V969P)

    SARS S protein (R667A, K968P, V969P), His Tag on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.

    SARS-CoV S Recombinant Protein (R667A, K968P, V969P)

    Immobilized Human ACE2, Fc Tag at 2 ug/ml (100 uL/well) can bind SARS S protein (R667A, K968P, V969P), His Tag with a linear range of 2-20 ng/mL.

    • SARS S protein (R667A, K968P, V969P) [orb750333]

      Unconjugated

      95%

      136.3 kDa

      SARS S protein (R667A, K968P, V969P), His Tag (orb750333) is expressed from human 293 cells (HEK293). It contains AA Ser 14 - Pro 1195 (Accession # AAP13567.1 (R667A, K968P, V969P)). The recombinant protein is expressed with T4 fibritin trimerization motif and a polyhistidine tag at the C-terminus. Proline substitutions (K968P, V969P) and alanine substitutions (R667A) are introduced to stabilize the trimeric prefusion state of SARS-CoV S protein and abolish the furin cleavage site, respectively.

      100 μg, 1 mg
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