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SARS-CoV-2 Spike RBD Protein (L452Q & F490S, His & Avi), Biotinylated
Catalog Number: orb1953534
| Catalog Number | orb1953534 |
|---|---|
| Category | Proteins |
| Description | SARS-CoV-2 Spike RBD Protein (L452Q & F490S, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 28.31 kDa and the accession number is A0A6G7K2L4. |
| Tag | C-His-Avi |
| Purity | 98.00% |
| MW | 28.31 kDa (predicted); 37.59 kDa (reducing condition, due to glycosylation) |
| UniProt ID | A0A6G7K2L4 |
| Expression System | HEK293 Cells |
| Biological Origin | SARS-CoV-2 |
| Biological Activity | SARS-CoV-2 Spike RBD Protein (L452Q & F490S, His & Avi), Biotinylated is expressed in HEK293 mammalian cells with His and Avi tag. The predicted molecular weight is 28.31 kDa and the accession number is A0A6G7K2L4. |
| Expression Region | A DNA sequence encoding the SARS-CoV-2 Spike RBD (YP_009724390.1, with mutations L452Q, F490S) (Arg319-Phe541) was expressed with a C-terminal polyhistidine tag followed by an AVI tag. The expressed protein was biotinylated in vivo by the Biotin-Protein ligase (BirA enzyme) which is co-expressed. The mutations were identified in the SARS-CoV-2 variant (known as C.37) which emerged in the Peru. Predicted N terminal: Arg 319 |
| Storage | -20°C |
| Note | For research use only |
| Application notes | A Certificate of Analysis (CoA) containing reconstitution instructions is included with the products. Please refer to the CoA for detailed information. |
| Expiration Date | 6 months from date of receipt. |
