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SARS-CoV-2 RBD Potent Neutralizing Antibody, monoclonal, Chimeric
Catalog Number: orb1274220
| Catalog Number | orb1274220 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 RBD Potent Neutralizing Antibody, monoclonal, Chimeric |
| Clonality | Recombinant |
| Tested applications | ELISA, WB |
| Reactivity | Mouse |
| Isotype | Human IgG1/kappa |
| Immunogen | Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) is a chimeric monoclonal antibody combining the constant domains of the human IgG1 molecule with mouse variable regions. Pseudovirus assay shows that this antibody has potent neutralizing activity against pseudovirus bearing SARS-CoV-2 Spike protein. |
| Concentration | batch dependent |
| Form/Appearance | Lyophilized |
| Conjugation | Unconjugated |
| Target | S |
| UniProt ID | P0DTC2 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | Lyophilized from 0.22 μm filtered solution in PBS, pH 7.4 . Normally trehalose is added as protectant before lyophilization. |
| Alternative names | spike glycoprotein Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 on SDS-PAGE under reducing (R) condition. The gel was stained overnight with Coomassie Blue. The purity of the protein is greater than 95%.
Immobilized SARS-CoV-2 S protein RBD, His Tag at 1 ug/mL (100 uL/well) can bind Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 (AM128) with a linear range of 0.05-3.13 ng/mL (QC tested).
Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 was detected by SARS-CoV-2 Inhibitor screening Kit with a half maximal inhibitory concentration (IC50) of 1.197 ug/mL (Routinely tested).
Serial dilutions of Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody was incubated with SARS-CoV-2 pseudotyped virus at 37C for 1 hour. Afterward, Huh-7 cells were added, followed by incubation at 37C for 24h. Chemiluminescence detection was performed and the virus neutralization titers (IC50) is 0.03255 nM using the Reed-Muench method.
Loaded Anti-SARS-CoV-2 RBD Potent Neutralizing Antibody, Chimeric mAb, Human IgG1 on AHC Biosensor, can bind SARS-CoV-2 S1 protein, His Tag with an affinity constant of 4.42 nM as determined in BLI assay (ForteBio Octet Red96e) (Routinely tested).
