SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10A1]

• Catalog Number: orb1239048
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Spike 681P Antibody [8G10A1]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 8G10A1
• Tested applications: ELISA, WB
• Reactivity: Virus
• Isotype: IgG3
• Immunogen: Anti-SARS-CoV-2 (COVID-19) Spike 681P antibody (orb1239048) was raised against a peptide corresponding to 12 amino acids near the center of SARS-CoV-2 Spike protein. The immunogen is located within 650-700 amino acids of the SARS-CoV-2 Spike protein.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: S
• UniProt ID: P0DTC2
• NCBI: QHD43416
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Anti-SARS-CoV-2 Spike 681P antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: SARS-CoV-2 Spike 681P antibody: Severe acute respi
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

ELISA Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Coating Antigens: SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK), 2 μg/mL, incubated at 4°C overnight. Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239048, dilution: 1-3000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239048) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by ELISA.

WB Validation of Spike 681P Antibodies with SARS-CoV-2 Spike S1 Protein.Loading: 30 ng of SARS-CoV-2 spike S1 proteins: WT and alpha variant (B.1.1.7, UK). Detection Antibodies: SARS-CoV-2 Spike 681P antibody, orb1239048, 1 μg/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239048) detected WT spike S1 protein (orb1231589), but not alpha variant spike S1 protein by WB.

ELISA Validation of Spike 681P Antibodies with Mutant and WT Peptide.Coating Antigen: SARS-CoV-2 spike S1 peptides: WT (orb1231701) and alpha variant (B.1.1.7, UK) (orb1231664), 1 μg/mL, incubate at 4°C overnight. Detection Antibodies: SARS-CoV-2 681P antibody, orb1239048, dilution: 0.3-1000 ng/mL, incubated at RT for 1 hr. Secondary Antibodies: Goat anti-mouse HRP at 1:5000, incubated at RT for 1 hr. SARS-CoV-2 spike 681P antibody (orb1239048) detected WT peptide (681P, orb1231701), but not alpha variant spike S1 peptide (681H, orb1231664).