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Item 1 of 3
SARS-CoV-2 (COVID-19) ORF9c Antibody
Catalog Number: orb1239056
| Catalog Number | orb1239056 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 (COVID-19) ORF9c Antibody |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, WB |
| Reactivity | Virus |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19) ORF9c antibody (orb1239056) was raised against a peptide corresponding to 16 amino acids near the amino-terminus of SARS-CoV-2 (COVID-19) ORF9c protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) ORF9c protein. |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | Unconjugated |
| MW | 8kD |
| Target | ORF9c |
| UniProt ID | P0DTD3 |
| NCBI | P0DTD3 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles. |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) ORF9c antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | ORF9c protein, Uncharacterized protein 14, ORF14, Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c orb1239056, 1 µg/mL, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.
Western Blot Validation with SARS-CoV-2 (COVID-19) ORF9c Overexpressed 293 Cells. Loading: 10 µg per lane of 293 cell lysate from WT and SARS-CoV-2 (COVID-19) ORF9c transfected cells. Antibodies: SARS-CoV-2 (COVID-19) ORF9c, orb1239056, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.25 µg/mL and. Lane 2: 0.5 µg/mL.
ELISA Validation. Antibodies: SARS-CoV-2 (COVID-19) ORF9c Antibody, orb1239056. A direct ELISA was performed using SARS-CoV-2 ORF9c immunogen peptide (orb1231666) as coating antigen and the anti-SARS-CoV-2 (COVID-19) ORF9c antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.3 ng/mL to 1000 ng/mL.
