SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3866]

• Catalog Number: orb1273021
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) Nucleocapsid Antibody [3866]
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3866
• Tested applications: ELISA, IF
• Reactivity: Virus
• Isotype: Mouse IgG1
• Immunogen: These newer COVID-19 nucleoprotein Mabs were developed using a recombinant, full-length nucleoprotein and then confirmed that they are reactive with the native virus itself.
• Concentration: batch dependent
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Coronavirus Nucleocapsid Antibody is in a phosphate saline buffer (0.01M, pH 7.2) containing 0.1% sodium azide preservative. No stabilizing proteins have been added.
• Alternative names: Coronavirus Nucleocapsid, SARS
• Note: For research use only
• Application notes: Suggested starting ranges are 1:10-1:50 for IFA and 1:20-1:200 for ELISA. BEST ELISA pairs (capture-conjugate): clones 3865-3866 / 3869-3866
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Nucleocapsid in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) Nucleocapsid antibody (orb1273021, 1 ug/ml). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 Nucleocapsid protein was observed in the macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1274175. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 2 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1274175, 0.1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1273021 and orb1239996. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1273021, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1239996, 1 ug/ml) as the detection antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 6.9 ng

Sandwich ELISA for SARS-CoV-2 (COVID-19) Matched Pair Nucleocapsid Antibodies. Antibodies: SARS-CoV-2 (COVID-19) Nucleocapsid Antibodies, orb1274176 and orb1273021. A sandwich ELISA was performed using SARS-CoV-2 Nucleocapsid antibody (orb1274176, 5 ug/ml) as capture antibody, the Nucleocapsid recombinant protein as the binding protein (orb1231585), and the anti-SARS-CoV-2 Nucleocapsid antibody (orb1273021, 1 ug/ml) as the detection antibody. Secondary: Goat anti-mouse IgG HRP conjugate at 1:20000 dilution. Detection range is from 0.03 ng to 300 ng. EC50 = 2.36 ng