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SARS-CoV-2 (COVID-19) Envelope Antibody (HRP)
Catalog Number: orb1239043
| Catalog Number | orb1239043 |
|---|---|
| Category | Antibodies |
| Description | SARS-CoV-2 (COVID-19) Envelope Antibody (HRP) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Tested applications | ELISA, IHC |
| Reactivity | Virus |
| Isotype | IgG |
| Immunogen | Anti-SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope antibody (HRP) (3531) was raised against a peptide corresponding to 10 amino acids near the amino terminus of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope protein. The immunogen is located within the first 50 amino acids of SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope. |
| Concentration | 1 mg/mL |
| Form/Appearance | Liquid |
| Conjugation | HRP |
| Target | E |
| UniProt ID | QHD43418 |
| NCBI | QHD43418 |
| Storage | SARS-CoV-2°COVID-19) Envelope antibody can be stored at 4 ˚ C for three months and -20 ˚ C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. |
| Buffer/Preservatives | SARS-CoV-2 (COVID-19) Envelope Antibody is supplied in PBS containing 0.02% sodium azide. |
| Alternative names | SARS-CoV-2 (COVID-19, 2019-nCoV) Envelope Antibody Read more... |
| Note | For research use only |
| Application notes | IHC: 2 μg/mL Antibody validated: Immunohistochemistry in COVID-19 patient samples. All other applications and species not yet tested. |
| Expiration Date | 12 months from date of receipt. |
Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) Envelope Protein in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti- SARS-CoV-2 (COVID-19) envelope antibody (orb1239043, 2 ug/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 °C. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 envelope protein was observed in macrophages of COVID-19 patient lung, but not in non-COVID-19 patient lung.
ELISA Validation. Coating Antigen: immunogen peptide, orb1236593, 10 ug/mL, incubate at 4 °C overnight. Detection Antibodies: SARS-CoV-2 Spike antibody, orb1239043 or orb1239971, dilution: 0.5-1000 ng/mL, incubate at RT for 1 hr. orb1239971 was detected by anti-rabbit HRP-conjugated secondary antibodies at 1:10000, incubate at RT for 1 hr.
