SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody

• Catalog Number: orb1239073
• Category: Antibodies
• Description: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Virus
• Isotype: IgG
• Immunogen: Anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody (orb1239073) was raised against a peptide corresponding to 16 amino acids near the amino terminus of SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein. The immunogen is located within the first 50 amino acids of the SARS-CoV-2 (COVID-19) 3CL-Pro (NSP5) protein.
• Concentration: 1 mg/mL
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: 3CL-PRO
• UniProt ID: P0DTC1
• NCBI: YP_009742612.1
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: 3C-like proteinase, 3CLp, nsp5, Non-structure prot
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Immunohistochemistry Validation of SARS-CoV-2 (COVID-19) 3L-PRO in COVID-19 Patient Lung. Immunohistochemical analysis of paraffin-embedded COVID-19 patient lung tissue using anti-SARS-CoV-2 (COVID-19) 3L-PRO (NSP5) antibody (orb1239073, 0.5 µg/mL). Tissue was fixed with formaldehyde and blocked with 10% serum for 1 h at RT; antigen retrieval was by heat mediation with a citrate buffer (pH6). Samples were incubated with primary antibody overnight at 4 ˚C. A goat anti-rabbit IgG H&L (HRP) at 1/250 was used as secondary. Counter stained with Hematoxylin. Strong signal of SARS-COV-2 3L-PRO (NSP5) protein was observed in macrophage of COVID-19 patient lung, but not in non-COVID-19 patient lung.

ELISA Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Antibody, orb1239073. A direct ELISA was performed using SARS-CoV-2 3CL-PRO (NSP5) recombinant protein (orb1231584) as coating antigen and the anti-SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) antibody as the capture antibody. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:20000 dilution. Detection range is from 1 ng/mL to 3000 ng/mL.

Western Blot Validation with SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) Protein. Loading: 30 ng per lane of SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5) recombinant protein (orb1231584). Antibodies: SARS-CoV-2 (COVID-19) 3CL-PRO (NSP5), orb1239073, 1 h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution. Lane 1: 0.5 µg/mL and. Lane 2: 1 µg/mL.