SARS-CoV-2 (2019-nCoV) Spike S1 Mouse Monoclonal Antibody

• Catalog Number: orb1035971
• Category: Antibodies
• Description: SARS-CoV-2 (2019-nCoV) Spike S1 Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 5E12
• Tested applications: WB
• Predicted Reactivity: Virus
• Reactivity: Mouse, Virus
• Isotype: IgG1
• Immunogen: Recombinant SARS-CoV-2 Spike S1 Protein (14-685/1213aa)
• Concentration: Lot Dependent
• Dilution range: WB=1:500-2000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 140 kDa
• Target: S
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Alternative names: SARS-CoV-2 spike protein; 2019-nCOV Spike protein;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Sample: _x000B_Lane 1: SARS-CoV-2 Spike RBD Protein (WT) at 500 ng, _x000B_Lane 2: SARS-CoV-2 Spike S1 Protein (E484Q, L452R, D614G, P681R) at 500 ng, _x000B_Lane 3: SARS-CoV-2 Spike S1 Protein (D80A, D215G, del241/243, K417N, E484K, N501Y, D614G) at 500 ng, _x000B_Lane 4: SARS-CoV-2 Spike S1 Protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y) at 500 ng, Lane 5: SARS-CoV-2 Spike S1 Protein (WT) at 500 ng_x000B_. Primary: Anti-SARS-CoV-2 Spike S1 Protein at 1/1000 dilution_x000B_, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution_x000B_Predicted band size: 27kDa /77.2 kDa_x000B_, Observed band size: 35kDa/114 kDa.

Sample: Lane 1: SARS-CoV-2 S1 Protein (E484Q, L452R, D614G, P681R) at 500 ng, Lane 2: SARS-CoV-2 S1 Protein (D80A, D215G, del241/243, K417N, E484K, N501Y, D614G) at 1.5 ng, Lane 3: SARS-CoV-2 S1 Protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y) at 500 ng, Lane 4: SARS-CoV-2 S1 Protein (His-Avi, HEK293) at 500 ng, Primary: Mouse Anti-SARS-CoV-2 Spike S1 Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 78kD, Observed band size: kD.

Sample: Lane 1: SARS-CoV-2 S1 Protein (E484Q, L452R, D614G, P681R) at 500 ng, Lane 2: SARS-CoV-2 S1 Protein (L18F, T20N, P26S, D138Y, R190S, K417T, E484K, N501Y, D614G, H655Y) at 500 ng, Primary: Mouse Anti-SARS-CoV-2 Spike S1 Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 77kD, Observed band size: kD.

Sample: Lane 1: SARS-CoV-2 S1 Protein (His-Avi, HEK293) at 500 ng, Primary: Mouse Anti-SARS-CoV-2 Spike S1 Protein Antibody at 1/1000 dilution, Secondary: IRDye800CW Goat Anti-Mouse IgG at 1/20000 dilution, Predicted band size: 78kD, Observed band size: kD.