SARS-CoV-2 (2019-nCoV) Spike Neutralizing Mouse Monoclonal Antibody

• Catalog Number: orb1048253
• Category: Antibodies
• Description: SARS-CoV-2 (2019-nCoV) Spike Neutralizing Mouse Monoclonal Antibody
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 7H3
• Tested applications: ELISA
• Predicted Reactivity: Virus
• Reactivity: Virus
• Isotype: IgG3
• Immunogen: Recombinant SARS-CoV-2 Spike S1 Protein (14-685/1213aa)
• Concentration: Lot Dependent
• Dilution range: ELISA=1:5000-10000
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: 140 kDa
• Target: S
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 0.01M PBS (pH7.4).
• Alternative names: SARS-CoV-2 spike protein; 2019-nCOV Spike protein;
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Direct ELISA was used to detect the binding ability of anti-RBD monoclonal antibody to RBD domain proteins of different SARS-CoV-2 Mutant Strains. Immobilized SARS-CoV-2 RBD proteins, at 2 µg/ml (100 ul/Well) can bind Anti-RBD monoclonal antibody-HRP at 1 ug/ml (100 ul/Well).

The ACE2-coated plate is incubated with SARS-CoV-2 Spike RBD-HRP (WT) and Anti-SARS-CoV-2 Spike RBD Neutralizing antibody. Percent inhibition is calculated based on the OD value by inhibiting RBD: ACE2 interaction.