SAE1 antibody

• Catalog Number: orb18563
• Category: Antibodies
• Description: Goat polyclonal antibody to SAE1
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF
• Reactivity: Bovine, Canine, Human
• Dilution range: ELISA: 1:8000, WB: 1 μg/ml
• Conjugation: Unconjugated
• MW: 38.4
• Target: SAE1 / AOS1
• Entrez: 10055
• Protein Sequence: MKGNGIVECLGPK
• RRID: AB_10755561
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti SAE1 antibody, anti AOS1 antibody, anti SUA1
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 40kDa band observed in Jurkat and 293 cell lysates (predicted MW of 40kDa according to NP_005491). Recommended for use at 1 μg/ml.
• Expiration Date: 12 months from date of receipt.

orb18563 staining (1ug/ml) of Jurkat lysate (RIPA buffer, 30ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb18563 Immunofluorescence analysis of paraformaldehyde fixed A431 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb18563 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb18563 Flow cytometric analysis of paraformaldehyde fixed A431 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.