S100A9 antibody

• Catalog Number: orb20117
• Category: Antibodies
• Description: Goat polyclonal antibody to S100A9
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, FC, IF, IHC, WB
• Reactivity: Human
• Dilution range: ELISA: 1:8000, WB: 0.5-2 μg/ml, IHC-P: 2.5ug/ml
• Conjugation: Unconjugated
• MW: 13.2
• Target: S100A9
• Entrez: 6280
• Protein Sequence: DTNADKQLSFEEF
• RRID: AB_10750615
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti S100A9 antibody, anti S100 calcium binding pr
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.IHC: In paraffin embedded Human Lung shows nuclear staining in select cells, Recommended concentration, 2-4ug/ml.WB: Approx 16kDa band observed in Human Peripheral Blood Mononucleocytes lysates (calculated MW of 13.2kDa according to NP_002956.1). The observed molecular weight corresponds to earlier findings (~15kDa) in literature with different antibodies (McCormick et al, Biol Chem. 2005 Dec 16;280(50):41521-9.; PMID: 16216873). Recommended concentration: 0.3-1 μg/ml.
• Expiration Date: 12 months from date of receipt.

Immunohistochemical staining of paraffin embedded Human Lung using S100A9 antibody

Western blot analysis of Human Peripheral Mononucleocytes lysate using S100A9 antibody

Immunohistochemical staining of Human Liver using S100A9 antibody

orb20117 (1 µg/mL) staining of Human Bone Marrow (A) and (0.5 µg/mL) Gastrointestinal cancer (B) lysate and negative control HepG2 (C) lysate. 5 µg protein in RIPA buffer). Detected by chemiluminescence.

orb20117 (2.5 µg/mL) staining of paraffin embedded Human Lung. Steamed antigen retrieval with citrate buffer pH 6, AP-staining.

orb20117 Immunofluorescence analysis of paraformaldehyde fixed MCF7 cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing cytoplasmic and nuclear staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb20117 Immunofluorescence analysis of paraformaldehyde fixed U2OS cells, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).

orb20117 Flow cytometric analysis of paraformaldehyde fixed MCF7 cells (blue line), permeabilized with 0.5% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (1 µg/mL). IgG control: Unimmunized goat IgG (black line) followed by Alexa Fluor 488 secondary antibody.

orb20117 (7 µg/mL) staining of paraffin embedded Human Spleen. Heat induced antigen retrieval with citrate buffer pH 6, HRP-staining.

orb20117 Negative Control showing staining of paraffin embedded Human Spleen, with no primary antibody.

orb20117 Immunofluorescence analysis of paraformaldehyde fixed THP-1 cells immobilized on ShifixTM coverslip, permeabilized with 0.15% Triton. Primary incubation 1 hr (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL), showing nuclear and cytoplasmic staining. The nuclear stain is DAPI (blue). Negative control: Unimmunized goat IgG (10 µg/mL) followed by Alexa Fluor 488 secondary antibody (2 µg/mL).