S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

• Catalog Number: orb750899
• Category: Antibodies
• Description: Mouse monoclonal antibody against S100A4 conjugated to Biotin
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A4/1481
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Concentration: Purified Ab conjugated to Biotin
• Dilution range: Flow Cytometry (5ul/million cells); Western Blot (2-4ug/ml); Immunofluorescence (2-4ug/ml at RT);,Immunohistochemistry (Formalin-fixed) (2-4ug/ml at RT);,(Staining of formalin-fixed tissues requires heating tissue sections in 10mM Tris with 1mM EDTA, pH 9.0, for 45 min at 95°C followed by cooling at RT for 20 minutes),Optimal dilution for a specific application should be determined.
• Form/Appearance: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 10-12kDa
• Target: S100A4
• Entrez: 6275
• UniProt ID: P26447
• Expression System: Bioreactor
• Storage: Antibody with azide - store at 2 to 8 °C. Antibody without azide - store at -20 to -80 °C. Antibody is stable for 24 months. Non-hazardous. No MSDS required.
• Buffer/Preservatives: Antibody purified from Bioreactor Concentrate by Protein A/G and conjugated to Biotin. Prepared in 10mM PBS with 0.05% rAlbumin and 0.05% azide.
• Alternative names: Anti-S100 calcium-binding protein A4 antibody, ant
• Note: For research use only
• Application notes: Positive Control: HeLa, A549 or A375 cells. Human placenta tissue.
• Expiration Date: 12 months from date of receipt.