S100A4 / Metastasin / Calvasculin (Marker of Tumor Metastasis) Antibody

• Catalog Number: orb388628
• Category: Antibodies
• Description: Mouse monoclonal antibody to S100A4
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A4/1481
• Tested applications: FC, IF, IHC, WB
• Reactivity: Human, Mouse
• Isotype: IgG1
• Immunogen: Recombinant fragment (around aa 1-200) of human S100A4 protein (exact sequence is proprietary)
• Concentration: Purified Ab with BSA and Azide at 200ug/ml
• Dilution range: FACS: 0.5-1 μg/million cells, IF/ICC: 1-2 μg/ml, WB: 0.5-1 μg/ml, IHC-P: 0.25-0.5 μg/ml
• Form/Appearance: 200ug/ml of Ab produced in HEK293 cell mammalian-based expression system. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Conjugation: Unconjugated
• MW: 10-12kDa
• Target: S100A4
• Entrez: 6275
• UniProt ID: P26447
• Expression System: HEK293 cells
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: 200ug/ml of Ab purified from Bioreactor Concentrate by Protein A/G. Prepared in 10mM PBS with 0.05% rAlbumin & 0.05% azide. Also available WITHOUT rAlbumin & azide at 1.0mg/ml.
• Alternative names: S100A4; S100 calcium-binding protein A4; Calvascul
• Note: For research use only
• Application notes: S100A4 (Marker of Tumor Metastasis)
• Expiration Date: 12 months from date of receipt.

Western blot analysis of Hela, A459 and A375 Cell Lysate using S100A4 antibody

Immunohistochemical staining of human Placenta tissue using S100A4 antibody