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Catalog Number | orb1274487 |
---|---|
Category | Antibodies |
Description | S100A4 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 1-101 of human S100A4 (NP_002952.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 12kDa |
Target | S100A4 |
UniProt ID | P26447 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | S100A4, S100 calcium binding protein A4, 18A2, 42A Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:50 - 1:200 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of various cell lines, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Western blot analysis of extracts from normal (control) and S100A4 knockout (KO) HeLa cells, using S100A4 antibody (orb1274487) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Immunohistochemistry of paraffin-embedded rat brain using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse lung using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human tonsil using S100A4 antibody (orb1274487) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of PC12 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.
Immunofluorescence analysis of RAW264.7 cells using S100A4 antibody (orb1274487) at dilution of 1:100. Blue: DAPI for nuclear staining.
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ELISA, FC, ICC, IF, IHC, WB | |
Human | |
Rabbit | |
Polyclonal | |
Unconjugated |
FACS, IF, IHC-P, WB | |
Human, Mouse | |
Mouse | |
Recombinant | |
Unconjugated |
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