S100A4 Antibody / FSP1

• Catalog Number: orb750071
• Category: Antibodies
• Description: S100A4/FSP1 (Fibroblast specific protein 1) belongs to the S100 super-family of proteins containing 2 EF-hand calcium-binding domains. S100 genes include at least 25 members, including S100A1-S100A18, trichohyalin, filaggrin, repetin, S100P, and S100Z. S100A4 exerts its function via direct interaction with a number of proteins including p53, p63, non-muscle myosin IIA, a6b4 integrin, and liprin b1. S100A4 is overexpressed in highly metastatic cancers, which makes it useful as a marker of tumor progression.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: S100A4/1482
• Tested applications: FACS, IF, IHC-P, WB
• Reactivity: Human, Mouse
• Isotype: Mouse IgG1, kappa
• Immunogen: Human recombinant S100A4 protein was used as the immunogen for this S100A4 antibody.
• Dilution range: Flow cytometry: 1-2ug/million cells,Immunofluorescence: 1-2ug/ml,Western blot: 1-2ug/ml,Immunohistochemistry (FFPE): 1-2ug/ml for 30 min at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced) and 0.05% sodium azide
• Hazard Information: This S100A4 antibody is available for research use only.
• UniProt ID: P26447
• Storage: Store the S100A4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced) and 0.05% sodium azide
• Note: For research use only
• Application notes: The optimal dilution of the S100A4 antibody for each application should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

Western blot testing of human 1) HeLa, 2) A549 and 3) A431 cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

IHC testing of FFPE human placenta with S100A4 antibody (clone S100A4/1482). Required HIER: steam sections in 10mM citrate buffer, pH 6, for 10-20 min followed by cooling.

Western blot testing of human HeLa cell lysate with S100A4 antibody (clone S100A4/1482). Predicted molecular weight ~12 kDa.

Flow cytometry testing of human T98G cells with S100A4 antibody (clone S100A4/1482); Red=isotype control, Blue=S100A4 antibody.

Flow cytometry testing of human A549 cells with S100A4 antibody (clone S100A4/1482); Red=isotype control, Blue=S100A4 antibody.

SDS-PAGE analysis of purified, BSA-free S100A4 antibody (clone S100A4/1482) as confirmation of integrity and purity.

IF/ICC testing of fixed and permeabilized human HeLa cells with FSP1 antibody (clone S100A4/1482, green) and Reddot nuclear stain (red).