S100A12 antibody

• Catalog Number: orb2276340
• Category: Antibodies
• Description: S100A12 antibody validated for immunohistochemistry on 76 different Normal Tissues
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: Biorbyt-812M
• Tested applications: IHC
• Reactivity: Human
• Isotype: IgG1, kappa
• Dilution range: 1:100-1:200
• Conjugation: Unconjugated
• UniProt ID: P80511
• Storage: Antibody with azide – store at 2 to 8°C. Antibody without azide – store at -20 to -80°C. Antibody is stable for 24 months. Non- hazardous. No MSD required.
• Alternative names: CAAF1, CAGC, CGRP, ENRAGE, MRP6, p6
• Note: For research use only
• Application notes: Positive Control: Spleen: A significant number of inflammatory cells should show a strong S100A12 immunostaining while the majority of cells remain completely negative. Negative Control: Colon: All epithelial and muscular cells must not show any S100A12 immunostaining. Few scattered S100A12 positive inflammatory cells can occur (mostly within blood vessels) Cellular Localization: Intracellular, Secreted Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining). Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7,8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
• Expiration Date: 12 months from date of receipt.

Colon Colorectal adenocarcinoma showing numerous S100A12 positive granulocytes in the stroma and within tumor glands.

Spleen S100A12 immunostaining is seen in granulocytes of mainly the red pulp.

Stomach Gastric adenocarcinoma densly infiltrated by S100A12 positive granulocytes.