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Catalog Number | orb1474273 |
---|---|
Category | Antibodies |
Description | Mouse monoclonal antibody to S100-A10 |
Species/Host | Mouse |
Clonality | Monoclonal |
Tested applications | IF, IP, WB |
Reactivity | Human, Mouse, Rat |
Immunogen | Purified recombinant mouse S100A10 protein fragments expressed in E.coli. |
Dilution range | WB (1/500 - 1/1000), IF/IC (1/50 - 1/100), IP (1/10 - 1/50) |
Form/Appearance | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Conjugation | Unconjugated |
Target | S100a10 |
Entrez | 81778, 20194 |
UniProt ID | P60903, P08207, P05943 |
Source | Mouse |
Storage | Shipped at 4°C. Upon delivery aliquot and store at -20°C for one year. Avoid freeze/thaw cycles. |
Buffer/Preservatives | Liquid in PBS containing 50% glycerol, 0.5% rAlbumin and 0.02% sodium azide, pH 7.3. |
Alternative names | ANX2LG; CAL1L; CLP11; Protein S100-A10; Calpactin Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of S100-A10 expression in C6 (A), NIH3T3 (B), Hela (C) whole cell lysates. (Predicted band size: 11 kD; Observed band size: 11 kD)
Immunofluorescent analysis of S100-A10 staining in HeLa cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a hidified chamber. Cells were washed with PBST and incubated with a AF488-conjugated secondary antibody (green) in PBS at room temperature in the dark. DAPI was used to stain the cell nuclei (blue).
ELISA, FC, ICC, IF, IHC, WB | |
Human, Monkey, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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