RUVBL1 monoclonal antibody (M01), clone 3G4-1F8

• Catalog Number: orb2291863
• Category: Antibodies
• Description: Mouse monoclonal antibody raised against a full length recombinant RUVBL1.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: 3G4-1F8
• Tested applications: ELISA, IF, PLA, WB
• Reactivity: Human
• Isotype: IgG2a kappa
• Immunogen: RUVBL1 (AAH02993, 1 a.a. ~ 456 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa.
• Conjugation: Unconjugated
• Protein Sequence: MKIEEVKSTTKTQRIASHSHVKGLGLDESGLAKQAASGLVGQENAREACGVIVELIKSKKMAGRAVLLAGPPGTGKTALALAIAQELGSKVPFCPMVGSEVYSTEIKKTEVLMENFRRAIGLRIKETKEVYEGEVTELTPCETENPMGGYGKTISHVIIGLKTAKGTKQLKLDPSIFESLQKERVEAGDVIYIEANSGAVKRQGRCDTYATEFDLEAEEYVPLPKGDVHKKKEIIQDVTLHDLDVANARPQGGQDILSMMGQLMKPKKTEITDKLRGEINKVVNKYIDQGIAELVPGVLFVDEVHMLDIECFTYLHRALESSIAPIVIFASNRGNCVIRGTEDITSPHGIPLDLLDRVMIIRTMLYTPQEMKQIIKIRAQTEGINISEEALNHLGEIGTKTTLRYSVQLLTPANLLAKINGKDSIEKEHVEEISELFYDAKSSAKILADQQDKYMK
• NCBI: AAH02993
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: In 1x PBS, pH 7.4
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Detection limit for recombinant GST tagged RUVBL1 is approximately 0.3 ng/ml as a capture antibody.

Immunofluorescence of monoclonal antibody to RUVBL1 on HeLa cell. [antibody concentration 10 ug/ml]

Proximity Ligation Analysis of protein-protein interactions between MAP3K7 and RUVBL1. HeLa cells were stained with anti-MAP3K7 rabbit purified polyclonal 1:1200 and anti-RUVBL1 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction complex, and nuclei were counterstained with DAPI (blue).

RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Western Blot analysis of RUVBL1 expression in human pancreas.

Western Blot analysis of RUVBL1 expression in transfected 293T cell line by RUVBL1 monoclonal antibody (M01), clone 3G4-1F8. Lane 1: RUVBL1 transfected lysate (Predicted MW: 50.2 KDa). Lane 2: Non-transfected lysate.

Western Blot detection against Immunogen (75.9 KDa).