RRM2B p53R2 antibody

• Catalog Number: orb345677
• Category: Antibodies
• Description: RRM2B p53R2 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC, IP, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Human RRM2B/p53R2 antibody was prepared by repeated immunizations with a synthetic peptide corresponding to a region near the N-terminus of human RRM2B1 protein. A residue of cysteine was added to facilitate coupling.
• Concentration: 0.82 mg/mL
• Dilution range: ELISA: 1:3,000, IHC: User Optimized, IF: User Optimized, IP: User Optimized, WB: 1 µg/mL
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-Human RRM2B/p53R2 is directed against RRM2B and reacts with the RRM2B from human tissues. Based on sequence we expect this antibody to react as well with RRM2B from other species. Based on 100% homology to the immunizing sequence, one may expect reactivity to chimpanzee and orangutan; based on 93% homology, reactivity to macaque, and 92% to pig and Drosophila.
• Conjugation: Unconjugated
• UniProt ID: Q7LG56
• NCBI: 42544136
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-RRM2B p53R2 antibody, rabbit anti-RRM2
• Note: For research use only
• Application notes: RRM2B/p53R2 antibody is tested in Western Blot and ELISA and suitable for IP, IHC, and IF. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 41 kDa in size corresponding to RRM2B1 by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's affinity purified anti-RRM2B antibody shows detection of recombinant (lanes 1 and 3) and endogenous protein (lanes 1 to 4) in whole cell extracts from transfected 293T. Lane 1 contains purified recombinant human p53R2. Lane 2 contains 293T cells transfected with control vector. Lane 3 contains 293T transfected with p53R2-myc. Lane 4: 293T transfected with ScRNA. Lane 5: 293T transfected with p53R2 SiRNA The band at 40.7 kDa, indicated by the bottom arrowhead, corresponds to the expected molecular weight of endogenous RRM2B. The band with the middle arrow corresponds to myc-tagged p53R2 at 41.9 kDa. The highest band at 44.3 kDa corresponds to recombinant p53R2. Primary antibody was diluted to 1 µg/mL and incubated overnight at 4°C. ECL detection was used.