RREB1 antibody

• Catalog Number: orb344631
• Category: Antibodies
• Description: RREB1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, WB
• Reactivity: Mouse
• Isotype: IgG
• Immunogen: Anti-RREB1 antibody was prepared from whole rabbit serum produced by repeated immunizations with a recombinant protein corresponding to mouse RREB1 protein.
• Concentration: 1.0 mg/ml
• Dilution range: ELISA: 1:2,000 - 1:15,000, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Anti-RREB1 antibody is directed against the mouse RREB1 protein. The product was Protein A purified from monospecific antiserum by immunoaffinity purification. Reactivity against homologues from other sources is not known.
• Conjugation: Unconjugated
• UniProt ID: Q3UH06
• NCBI: AAX83010.1
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: rabbit anti-RREB1 Antibody, rabbit anti-RREB 1 Ant
• Note: For research use only
• Application notes: This Protein A purified antibody has been tested for use in ELISA and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a predominant band approximately 90-180 kDa in size corresponding to RREB1 by western blotting in the appropriate cell lysate or extract. Multiple bands seen in the above western blot may indicate cross-reactive isoforms or truncated protein products.
• Expiration Date: 12 months from date of receipt.

Western blot using Biorbyt's Protein A Purified anti-RREB1 antibody shows detection of a predominant band believed to be RREB1 in various cell lysates (1 - HEK293, 2 - RFP-RREB transfected HEK293, 3 - M460 and 4 - T1165). All lysates were loaded at 20 µg per lane and separated by SDS-PAGE. After transfer to nitrocellulose, the membrane was probed with the primary antibody diluted to 1:1000. The membrane was washed and reacted with IRDye800 conjugated Gt-a-Rabbit IgG [H&L] MX.