RPS6KB1 Antibody

• Catalog Number: orb1247899
• Category: Antibodies
• Description: RPS6KB1 Antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Predicted Reactivity: Bovine, Canine
• Reactivity: Human, Mouse, Rat
• Immunogen: The immunogen for this antibody is: C-MISKRPEHLRMNL
• Concentration: 500 ug/mL
• Dilution range: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in lysates of Human Placenta, of Rat Brain and of cell line NIH3T3 (calculated MW of 59.1kDa according to NP_003152.1). Recommended concentration: 0.05-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. Recommended concentration: 2-4ug/ml.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: RPS6KB1
• UniProt ID: P23443
• NCBI: NP_003152.1
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: RPS6KB1, p70-S6K, ribosomal protein S6 kinase, 70k
• Note: For research use only
• Application notes: Peptide ELISA: antibody detection limit dilution 1:8000.Western Blot:Approx 65kDa band observed in lysates of Human Placenta, of Rat Brain and of cell line NIH3T3 (calculated MW of 59.1kDa according to NP_003152.1). Recommended concentration: 0.05-0.1ug/ml.Immunohistochemistry:In paraffin embedded Human Kidney shows staining in the cytoplasm-rich PCT while less so in the DCT and no signals in the glomerulus. Recommended concentration: 2-4ug/ml.
• Expiration Date: 12 months from date of receipt.

orb1247899 staining (0.05 ug/ml) of Human Placenta lysate (RIPA buffer, 35 ug total protein per lane). Primary incubated for 1 hour. Detected by western blot using chemiluminescence.

orb1247899 (2 ug/ml) staining of paraffin embedded Human Kidney. Steamed antigen retrieval with citrate buffer pH 6, HRP-staining.

orb1247899 (1 ug/ml) staining of Rat Brain lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.

orb1247899 (0.1 ug/ml) staining of NIH3T3 cell lysate (35 ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.