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Catalog Number | orb1254434 |
---|---|
Category | Antibodies |
Description | RPS6KA3 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IF, IHC, WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | Recombinant fusion protein containing a sequence corresponding to amino acids 68-327 of human RPS6KA3 (NP_004577.1). |
Concentration | batch dependent |
Dilution range | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | Observed: 84kDa |
Target | RPS6KA3 |
UniProt ID | P51812 |
Storage | Store at -20˚°C. Avoid freeze / thaw cycles. |
Buffer/Preservatives | Liquid |
Alternative names | Ribosomal protein S6 kinase alpha-3, S6K-alpha-3, Read more... |
Note | For research use only |
Application notes | WB: 1:500 - 1:2000IHC: 1:50 - 1:200IF: 1:20 - 1:50 |
Expiration Date | 12 months from date of receipt. |
Western blot analysis of extracts of mouse lung, using RPS6KA3 antibody (orb1254434) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25 ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 90s.
Immunohistochemistry of paraffin-embedded rat kidney using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded human stomach using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunohistochemistry of paraffin-embedded mouse stomach using RPS6KA3 antibody (orb1254434) at dilution of 1:100 (40x lens).
Immunofluorescence analysis of HeLa cells using RPS6KA3 antibody (orb1254434). Blue: DAPI for nuclear staining.
ICC, IF, IHC, WB | |
Human, Mouse, Rat | |
Polyclonal | |
Unconjugated |
IF, IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC-P | |
Bovine, Canine, Equine, Gallus, Human, Mouse, Porcine, Rabbit, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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