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Item 1 of 3
RPL23 Antibody (Center)
Catalog Number: orb1937356
| Catalog Number | orb1937356 |
|---|---|
| Category | Antibodies |
| Description | Affinity Purified Rabbit Polyclonal Antibody (Pab) |
| Species/Host | Rabbit |
| Clonality | Polyclonal |
| Clone Number | RB31322 |
| Tested applications | IHC-P, WB |
| Predicted Reactivity | Porcine, Rabbit, Zebrafish |
| Reactivity | Human, Mouse, Rat |
| Isotype | Rabbit IgG |
| Dilution range | WB: 1:2000, WB: 1:1000, IHC-P: 1:10~50 |
| Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification. |
| Conjugation | Unconjugated |
| MW | 14865 Da |
| Target | This RPL23 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 49-78 amino acids from the Central region of human RPL23. |
| UniProt ID | P62829 |
| NCBI | NP_000969.1 |
| Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
| Alternative names | 60S ribosomal protein L23, 60S ribosomal protein L Read more... |
| Note | For research use only |
| Expiration Date | 12 months from date of receipt. |
All lanes: Anti-RPL23 Antibody (Center) at 1:2000 dilution. Lane 1: A431 whole cell lysate. Lane 2: U-2OS whole cell lysate. Lane 3: U-251 MG whole cell lysate. Lane 4: THP-1 whole cell lysate. Lane 5: NIH/3T3 whole cell lysate. Lane 6: mouse brain lysate. Lane 7: rat brain lysate. Lysates/proteins at 20 µg per lane. Secondary Goat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size: 15 kDa. Blocking/Dilution buffer: 5% NFDM/TBST.
RPL23 Antibody (Center) immunohistochemistry analysis in formalin fixed and paraffin embedded human stomach tissue followed by peroxidase conjugation of the secondary antibody and DAB staining. This data demonstrates the use of RPL23 Antibody (Center) for immunohistochemistry. Clinical relevance has not been evaluated.
RPL23 Antibody (Center) western blot analysis in mouse NIH-3T3 cell line lysates (35 ug/lane). This demonstrates the RPL23 antibody detected the RPL23 protein (arrow).
