RPL14 antibody

• Catalog Number: orb382013
• Category: Antibodies
• Description: Rabbit polyclonal antibody to RPL14.
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IF, WB
• Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant full length protein of human RPL14
• Dilution range: WB: 1:500-2000, IHC-P: 1:50-200, IF/ICC: 1:50-200
• Form/Appearance: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Conjugation: Unconjugated
• Target: RPL14
• Entrez: 9045
• UniProt ID: Q63507, P50914
• Source: Rabbit
• Storage: Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
• Buffer/Preservatives: Liquid in 0.42% Potassium phosphate, 0.87% Sodium chloride, pH 7.3, 30% glycerol, and 0.01% sodium azide.
• Alternative names: 60S ribosomal protein L14; CAG-ISL 7
• Note: For research use only
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RPL14 expression in Jurkat (A), HepG2 (B), mouse spleen (C), mouse ovary (D), rat kidney (E) whole cell lysates. (Predicted band size: 23 kD; Observed band size: 26 kD)

Immunofluorescent analysis of RPL14 staining in Hela cells. Formalin-fixed cells were permeabilized with 0.1% Triton X-100 in TBS for 5-10 minutes and blocked with 3% BSA-PBS for 30 minutes at room temperature. Cells were probed with the primary antibody in 3% BSA-PBS and incubated overnight at 4 °C in a humidified chamber. Cells were washed with PBST and incubated with a DyLight 594-conjugated secondary antibody (red) in PBS at room temperature in the dark.