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Catalog Number | orb1530485 |
---|---|
Category | Antibodies |
Description | RPA70 Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Between 350 and 400 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RPA70 |
UniProt ID | P27694 |
NCBI | NP_002936.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | RP-A; single-stranded DNA-binding protein; replica Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human RPA70 by western blot.Samples: Whole cell lysate from HeLa and from HEK293T cells. Antibody: Affinity purified rabbit anti-RPA70 antibody orb1530485 at 0.1 µg/ml.
Detection of mouse RPA70 by western blot.Samples: Whole cell lysate from NIH 3T3 cells. Antibody: Affinity purified rabbit anti-RPA70 antibody orb1530485 at 0.1 µg/ml.
Detection of mouse RPA70 by immunohistochemistry.Samples: FFPE serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti-RPA70 used at a dilution of 1:1, 000 (left) and 1:5, 000 (right).
Detection of human RPA70 by immunohistochemistry.Samples: FFPE serial sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti-RPA70 used at a dilution of 1:1, 000 (left) and 1:5, 000 (right).
Detection of human RPA70 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RPA70 antibody.
FC, IF, IHC, WB | |
Human, Monkey, Mouse | |
Mouse | |
Monoclonal | |
Unconjugated |
ICC, IP, WB | |
Human, Monkey, Mouse, Rat | |
Mouse | |
Monoclonal | |
Unconjugated |
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