RPA70 Antibody

• Catalog Number: orb1530484
• Category: Antibodies
• Description: RPA70 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Between 350 and 400
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:1,000 - 1:5,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RPA70
• UniProt ID: P27694
• NCBI: NP_002936.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RP-A; single-stranded DNA-binding protein; replica
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human RPA70 by western blot.Samples: Whole cell lysate from HeLa and from HEK293T cells. Antibody: Affinity purified rabbit anti-RPA70 antibody orb1530484 at 0.1 µg/ml.

Detection of mouse RPA70 by western blot.Samples: Whole cell lysate from NIH 3T3 cells. Antibody: Affinity purified rabbit anti-RPA70 antibody orb1530484 at 0.1 µg/ml.

Detection of human RPA70 by immunohistochemistry.Samples: FFPE serial sections of human ovarian carcinoma. Antibodies: Affinity purified rabbit anti-RPA70 Lot2 used at a dilution of 1:1, 000 (left) and 1:5, 000 (right).

Detection of mouse RPA70 by immunohistochemistry.Samples: FFPE serial sections of mouse teratoma. Antibodies: Affinity purified rabbit anti-RPA70 Lot2 used at a dilution of 1:1, 000 (left) and 1:5, 000 (right).

Detection of human RPA70 by western blot of immunoprecipitates.Samples: Whole cell lysate from HeLa cells. Antibodies: Affinity purified rabbit anti-RPA70 antibody.