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Catalog Number | orb500782 |
---|---|
Category | Antibodies |
Description | ROR Alpha antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human, Mouse, Rat |
Isotype | IgG |
Immunogen | KLH conjugated synthetic peptide derived from mouse ROR alpha |
Concentration | 1mg/ml |
Dilution range | WB=1:500-1000 |
Form/Appearance | Liquid |
Conjugation | Unconjugated |
MW | 59 kDa |
Target | ROR alpha |
UniProt ID | P35398 |
Storage | Shipped at 4°C. Store at -20 °C for one year. Avoid repeated freeze/thaw cycles. |
Buffer/Preservatives | Preservative: 15mM Sodium Azide, Constituents: 1% rAlbumin, 0.01M PBS, pH 7.4 |
Alternative names | NR1F1; Nuclear receptor ROR-alpha; Nuclear recepto Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Sample: A431(human) Cell Lysate at 40 ug Primary: Anti-ROR alpha (orb500782) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Sample: Muscle (Mouse) Lysate at 40 ug Primary: Anti-ROR alpha (orb500782) at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59 kD Observed band size: 59 kD
Paraformaldehyde-fixed, paraffin embedded (Rat testis); Antigen retrieval by boiling in sodium citrate buffer (pH6.0) for 15min; Block endogenous peroxidase by 3% hydrogen peroxide for 20 minutes; Blocking buffer (normal goat serum) at 37°C for 30min; Ant
Sample: Jurkat (Human) Cell Lysate at 40 ug Primary: Anti-ROR alpha(orb500782)at 1/300 dilution Secondary: IRDye800CW Goat Anti-Rabbit IgG at 1/20000 dilution Predicted band size: 59kD Observed band size: 59 kD
IF, IH, WB | |
Human, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, IF, IHC-P, WB | |
Human, Monkey | |
Rabbit | |
Polyclonal | |
Unconjugated |
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