ROCK1 Antibody

• Catalog Number: orb1239116
• Category: Antibodies
• Description: ROCK1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Rabbit
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: ROCK1 antibody was raised against a 15 amino acid synthetic peptide near the amino terminus of human ROCK1.The immunogen is located within the first 50 amino acids of ROCK1.
• Concentration: 1 mg/mL
• Dilution range: ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• Target: ROCK1
• UniProt ID: Q13464
• NCBI: NP_005397
• Storage: ROCK1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.
• Buffer/Preservatives: ROCK1 Antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: ROCK1 Antibody: ROCK-I, P160ROCK, Rho-associated p
• Note: For research use only
• Application notes: ROCK1 antibody can be used for detection of ROCK1 by Western blot at 1 μg/mL. Antibody can also be used for immunocytochemistry starting at 10 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of ROCK1 in 293 cell lysate with ROCK1 antibody at 1 µg/mL in (A) the absence and (B) the presence of blocking peptide.

Immunocytochemistry of ROCK1 in 293 cells with ROCK1 antibody at 10 µg/mL.

Immunofluorescence of ROCK1 in 293 cells with ROCK1 antibody at 20 µg/mL.