RNF7 antibody

• Catalog Number: orb20424
• Category: Antibodies
• Description: Goat polyclonal antibody to RNF7
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IHC, WB
• Reactivity: Human, Mouse
• Dilution range: ELISA: 1:8000, WB: 1-3 μg/ml, IHC-P: 5 μg/ml
• Conjugation: Unconjugated
• MW: 12.7; 9.64
• Target: RNF7
• Entrez: 9616
• Protein Sequence: DACLRCQAENKQE
• RRID: AB_10753550
• Storage: Aliquot and store at -20°C. Minimize freezing and thawing.
• Buffer/Preservatives: Supplied at 0.5 mg/ml in Tris saline, 0.02% sodium azide, pH 7.3 with 0.5% bovine serum albumin. Aliquot and store at -20°C. Minimize freezing and thawing.
• Alternative names: anti RNF7 antibody, anti ring finger protein 7 ant
• Note: For research use only
• Application notes: ELISA: Peptide ELISA: antibody detection limit dilution 1:8000.WB: Approx 11kDa band observed in Human Human Heart lysates (calculated MW of 12.7kDa according to NP_055060.1). Recommended concentration: 1-3 μg/ml. An additional band of 28kDa was consistently observed, however this band was not blocked by the immunizing peptide and it is therefore a non-specific signal. We call for caution when used for other assays than Western blot.
• Expiration Date: 12 months from date of receipt.

orb20424 (1ug/ml) staining of Human Heart lysate (35ug protein in RIPA buffer). Primary incubation was 1 hour. Detected by chemiluminescence.