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Catalog Number | orb1529845 |
---|---|
Category | Antibodies |
Description | RNA Polymerase II, Phospho (S2) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, IP, WB |
Reactivity | Human, Mouse |
Immunogen | Surrounding serine 2 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RNA Polymerase II, Phospho (S2) |
UniProt ID | P24928 |
NCBI | NP_000928.1 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polym Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho RNA Polymerase II (S2) by western blot.Samples: HeLa prepared using NETN buffer. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1529845 was at 0.04 µg/ml (upper panel).
Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2µg/ml).
Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:200 (1µg/ml).
Detection of Phospho RNA Polymerase II (S2) by WB of immunoprecipitates.Samples: HeLa cells.Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody for IP and Affinity purified rabbit anti-RNA Polymerase II antibody for IP.
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
IHC, IP, WB | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, ICC, IF, IHC, IP, WB | |
Human, Mouse, Rat | |
Rabbit | |
Monoclonal | |
Unconjugated |
ELISA, IF, IHC, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
IHC | |
Human, Mouse | |
Rabbit | |
Polyclonal | |
Unconjugated |
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