RNA Polymerase II, Phospho (S2) Antibody

• Catalog Number: orb1529844
• Category: Antibodies
• Description: RNA Polymerase II, Phospho (S2) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 2
• Concentration: 200 µg/ml
• Dilution range: WB - 1:2,000 - 1:10,000; IP - 2 - 10 µg/mg lysate; IHC - 1:200 - 1:1,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RNA Polymerase II, Phospho (S2)
• UniProt ID: P24928
• NCBI: NP_000928.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide
• Alternative names: RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polym
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RNA Polymerase II (S2) by western blot.Samples: HeLa prepared using NETN buffer. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1529844 was at 0.04 µg/ml (upper panel).

Detection of human Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:1000 (0.2µg/ml).

Detection of mouse Phospho-RNA Polymerase II (S2) by immunohistochemistry. Sample: FFPE section of mouse teratoma. Antibody: Affinity purified rabbit anti-Phospho-RNA Polymerase II (S2) used at a dilution of 1:200 (1µg/ml).

Detection of Phospho RNA Polymerase II (S2) by WB of immunoprecipitates.Samples: HeLa cells. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody for IP and Affinity purified rabbit anti-RNA Polymerase II antibody for IP.