RNA Polymerase II, Phospho (S2) Antibody

• Catalog Number: orb1523052
• Category: Antibodies
• Description: RNA Polymerase II, Phospho (S2) Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: IHC, IP, WB
• Reactivity: Human, Mouse
• Immunogen: Surrounding serine 2
• Concentration: 1000 µg/ml
• Dilution range: WB - 1:500 - 1:2,500; IP - 2 - 10 µg/mg lysate; IHC - 1:2,000 - 1:10,000. Epitope retrieval with citrate buffer pH 6.0 is recommended for FFPE tissue sections.
• Form/Appearance: Whole IgG
• Conjugation: Unconjugated
• Target: RNA Polymerase II, Phospho (S2)
• UniProt ID: P24928
• NCBI: NP_000928.1
• Storage: 2 - 8°C
• Buffer/Preservatives: Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide
• Alternative names: RPBh1; RPOL2; RpIILS; RPB1; RNA-directed RNA polym
• Note: For research use only
• Application notes: Format: Whole IgG
• Expiration Date: 12 months from date of receipt.

Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: RNA Polymerase II was immunoprecipitated using rabbit anti-RNA Polymerase II antibody. Following immunoprecipitation the product of the IP reaction was mock treated (-) or treated with phosphatases (+). Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1523052 was used for WB at 1 µg/ml (upper panel). To examine total RNA Polymerase II, the blot was stripped and then blotted with rabbit anti-RNA Polymerase II antibody (lower panel). Detection: Chemiluminescence with exposure times of 30 seconds (upper panel) and 15 seconds (lower panel).

Detection of human Phospho RNA Polymerase II (S2) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. orb1523052) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of mouse Phospho RNA Polymerase II (S2) by immunohistochemistry.Sample: FFPE section of mouse renal cell carcinoma. Antibody: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) (Cat. No. orb1523052) used at a dilution of 1: 5, 000 (0.2µg/ml). Detection: DAB

Detection of human Phospho RNA Polymerase II (S2) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) prepared using NETN buffer from 293T cells. Antibodies: Affinity purified rabbit anti-Phospho RNA Polymerase II (S2) antibody orb1523052 used for IP at 6 µg per reaction.