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Catalog Number | orb1929500 |
---|---|
Category | Antibodies |
Description | Purified Rabbit Polyclonal Antibody (Pab) |
Species/Host | Rabbit |
Clonality | Polyclonal |
Clone Number | RB8634 |
Tested applications | IHC-P, WB |
Reactivity | Human, Mouse |
Isotype | Rabbit IgG |
Antibody Type | Primary Antibody |
Dilution range | WB: 1:1000, WB: 1:1000, WB: 1:1000, IHC-P: 1:10~50 |
Form/Appearance | Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is prepared by Saturated Ammonium Sulfate (SAS) precipitation followed by dialysis against PBS. |
Conjugation | Unconjugated |
MW | 56887 Da |
Target | This RIPK3 antibody is generated from rabbits immunized with a KLH conjugated synthetic peptide between 61-91 amino acids from the Central region of human RIPK3. |
UniProt ID | Q9Y572 |
NCBI | NP_006862.2 |
Storage | Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles |
Alternative names | Receptor-interacting serine/threonine-protein kina Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Formalin-fixed and paraffin-embedded human lung carcinoma tissue reacted with RIP3 (RIPK3) antibody (Center), which was peroxidase-conjugated to the secondary antibody, followed by DAB staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated.
Western blot analysis of RIP3 (RIPK3) Antibody (Center) in Hela cell line lysates (35 ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Western blot analysis of RIP3 (RIPK3) Antibody (Center) in mouse cerebellum tissue lysates (35 ug/lane). RIPK3 (arrow) was detected using the purified Pab.
Western blot analysis of lysates from HT-29, K562 cell line (from left to right), using RIPK3 Antibody Center. Diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L (HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 35 ug per lane.