RING1B antibody

• Catalog Number: orb345298
• Category: Antibodies
• Description: RING1B antibody
• Species/Host: Goat
• Clonality: Polyclonal
• Tested applications: ELISA, IF, Multiplex Assay, WB
• Reactivity: Chinese Hamster, Human, Mouse
• Isotype: IgG
• Immunogen: RING1B Antibody was prepared from whole goat serum produced by repeated immunizations with a synthetic peptide corresponding to an internal region near aa 180-205 of human RING1B protein.
• Concentration: 1.1 mg/mL
• Dilution range: ELISA: 1:5,000 - 1:25,000, IF: 1:300, WB: 1:500 - 1:2,000
• Form/Appearance: Liquid (sterile filtered)
• Purity: Affinity purified Anti-RING1B antibody is directed against human RING1B protein. The product was affinity purified from monospecific antiserum by immunoaffinity purification. A BLAST analysis was used to suggest reactivity with this protein from human, chimpanzee, orangutan, mouse, rat, dog, bovine, frog and chicken based on 100% homology for the immunogen sequence. Expect cross reactivity with RING1B from zebrafish, as only a single amino acid residue changes within the immunogen sequence (92% positive by BLAST). Cross reactivity with RING1B homologues from other sources has not been determined.
• Conjugation: Unconjugated
• UniProt ID: Q99496
• NCBI: 6005747
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: goat anti-RING1B Antibody, RING finger protein BAP
• Note: For research use only
• Application notes: Anti-RING1B purified antibody has been tested for use in ELISA, immunofluorescence, and by western blot. Specific conditions for reactivity should be optimized by the end user. Expect a band approximately 38 kDa in size corresponding to RING1B by western blotting in the appropriate cell lysate or extract.
• Expiration Date: 12 months from date of receipt.

Immunofluorescence Microscopy of Goat anti-RING1B antibody. Tissue: human HeLa cells. Fixation: methanol and blocked with 0.2% fish scale gelatin for 1 hour at 25°C. Antigen retrieval: not required. Primary antibody: RING1B antibody at 1:300 for 20 minutes at 25°C. Secondary antibody: Alexa Fluor®488-conjugated Donkey anti-goat IgG secondary antibody at 1:500 for 45 min at RT. Localization: RING1B is nuclear and occasionally cytoplasmic. Staining: RING1B (RNF2) as green signal, Tubulin cytoplasm staining red, and DAPI (blue) nuclear counterstain.

Western blot using Biorbyt's Affinity Purified anti-RING1B antibody shows detection of a 38 kDa band corresponding to human RING1B. Lane 1: 3T3 whole cell lysates (p/n orb348714). Lane 2: U937 (p/n orb348704). Lane 3: Jurkat (p/n orb348674). Lane 4: mouse brain (p/n orb348715). Lane 5: CHO-K1. Approximately 20 µg of lysate was run on a SDS-PAGE and transferred onto nitrocellulose followed by reaction with a 1:500 dilution of anti-RING1B antibody incubated at room temperature. Signal was detected using standard techniques.