RHOT1 Antibody

• Catalog Number: orb1239136
• Category: Antibodies
• Description: RHOT1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, IF, IHC-P, WB
• Predicted Reactivity: Bovine, Gallus
• Reactivity: Human, Mouse, Rat
• Isotype: IgG
• Immunogen: RHOT1 antibody was raised against a 15 amino acid peptide near the amino terminus of human RHOT1.The immunogen is located within amino acids 40 - 90 of RHOT1.
• Concentration: 1 mg/mL
• Dilution range: RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 76 kDa Observed: 78 kDa
• Target: RHOT1
• UniProt ID: Q8IXI2
• NCBI: NP_001028740
• Storage: RHOT1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: RHOT1 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: Ras homolog family member T1, mitochondrial Rho GT
• Note: For research use only
• Application notes: RHOT1 antibody can be used for detection of RHOT1 by Western blot at 1 - 2 μg/ml. Antibody can also be used for immunohistochemistry starting at 5 μg/mL. For immunofluorescence start at 20 μg/mL.Antibody validated: Western Blot in rat samples; Immunohistochemistry in mouse samples and Immunofluorescence in mouse samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RHOT1 in rat brain tissue lysate with RHOT1 antibody at 1 µg/ml.

Immunohistochemistry of RHOT in mouse brain tissue with RHOT antibody at 5 µg/ml.

Immunofluorescence of RHOT in mouse brain tissue with RHOT antibody at 20 µg/ml.