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Catalog Number | orb1672040 |
---|---|
Category | Antibodies |
Description | RHO Antibody |
Clonality | Recombinant |
Clone Number | Rho 1D4 |
Tested applications | ELISA, IF, IHC, IP, RIA, WB |
Reactivity | Amphibian, Bovine, Human, Mouse, Rat, Zebrafish |
Isotype | IgG kappa |
Immunogen | Bleached bovine rod outer segment (ROS). |
Concentration | batch dependent |
Conjugation | Unconjugated |
Target | RHO |
UniProt ID | P08100 |
Storage | Store at 4°C for up to 3 months. For longer storage, aliquot and store at -20°C. |
Buffer/Preservatives | PBS with 0.02% Proclin 300. |
Alternative names | Rho-tag, Visual Purple Read more... |
Note | For research use only |
Expiration Date | 12 months from date of receipt. |
Western Blot using anti-Rhodopsin antibody Rho 1D4. Rat eye lysate (35 ug protein in RIPA buffer) was resolved on a 10% SDS PAGE gel and blots probed with the chimeric rsion of Rho 1D4 (orb1672040) at 0.0001 ug/ml before detection using an anti-rondary antibody. A primary incubation of 1h was used and protein was detected by chemiluminescence. The expected band size for monomeric rat (Rattus norvegicus) Rhodopsin is 39.0kDa- tho ugh this protein in known to form dimers and higher order multimers (c.f. Mansoor et al.- PMID: 16492772- PMID: 10798675). orb1672040 successfully detected monomeric- dimeric and multimeric Rhodopsin in rat eye lysate.
IHC-P, WB | |
Guinea pig, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IF, IHC-P, WB | |
Monkey | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
ELISA, ICC, IHC-Fr, IHC-P, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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