RFX1 Antibody

• Catalog Number: orb1238773
• Category: Antibodies
• Description: RFX1 Antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: ELISA, ICC, IF, WB
• Predicted Reactivity: Mouse
• Reactivity: Human
• Isotype: IgG
• Immunogen: RFX1 antibody was raised against an 18 amino acid peptide near the carboxy terminus of human RFX1.The immunogen is located within the last 50 amino acids of RFX1.
• Concentration: 1 mg/mL
• Dilution range: RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Form/Appearance: Liquid
• Conjugation: Unconjugated
• MW: Predicted: 108 kDa Observed: 125 kDa
• Target: RFX1
• UniProt ID: P22670
• NCBI: NP_002909
• Storage: RFX1 antibody can be stored at 4°C for three months and -20°C, stable for up to one year.
• Buffer/Preservatives: RFX1 antibody is supplied in PBS containing 0.02% sodium azide.
• Alternative names: RFX, MHC class II regulatory factor RFX, MHC class
• Note: For research use only
• Application notes: RFX1 antibody can be used for detection of RFX1 by Western blot at 1 - 2 μg/mL. Antibody can also be used for immunocytochemistry starting at 5 μg/mL.Antibody validated: Western Blot in human samples; Immunocytochemistry in human samples and Immunofluorescence in human samples. All other applications and species not yet tested.
• Expiration Date: 12 months from date of receipt.

Western blot analysis of RFX1 in HeLa cell lysate with RFX1 antibody at (A) 1 and (B) 2 µg/ml.

Immunocytochemistry of RFX1 in HeLa cells with RFX1 at 5 µg/mL.

Immunofluorescence of RFX1 in Hela cells with RFX1 antibody at 20 µg/mL. Green: RFX1 Antibody (orb1238773) Blue: DAPI staining.

Immunocytochemistry of RFX1 in Hela cells with RFX1 antibody at 5 µg/mL.