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Catalog Number | orb345913 |
---|---|
Category | Antibodies |
Description | RFP antibody (Biotin) |
Species/Host | Gallus |
Clonality | Polyclonal |
Tested applications | ELISA, IF, WB |
Reactivity | Other |
Isotype | IgY |
Immunogen | The immunogen is a Red Fluorescent Protein (RFP) fusion protein corresponding to the full length amino acid sequence (234aa) derived from the mushroom polyp coral Discosoma. |
Concentration | 1.0 mg/mL |
Dilution range | ELISA: 1:10,000, IF: 1:100, WB: 1:1,000 - 1:3,000 |
Form/Appearance | Lyophilized |
Purity | RFP Antibody was prepared from egg yolks by a multi-step process which includes filtration, delipidation, salt fractionation and extensive dialysis against the buffer stated above. RFP Antibody was tested by western blot and immunoelectrophoresis against anti-biotin, anti-chicken serum, and RFP. |
Conjugation | Biotin |
UniProt ID | Q9U6Y8 |
Storage | Store vial at 4° C prior to restoration. For extended storage aliquot contents and freeze at -20° C or below. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use. |
Buffer/Preservatives | 0.01% (w/v) Sodium Azide |
Alternative names | chicken anti-RFP antibody biotin conjugation, biot Read more... |
Note | For research use only |
Application notes | Anti-RFP is designed to detect recombinant RFP. This antibody has been tested to detect RFP by immunoblotting and immunofluorescence and is suitable for use in immunohistochemistry. Use either alkaline phosphatase or peroxidase conjugated polyclonal anti-RFP to detect RFP or RFP containing proteins on western blots. Optimal titers for applications should be determined by the researcher. This product shows optimal performance by western blot. |
Expiration Date | 12 months from date of receipt. |
Immunofluorescence Microscopy of Chicken Anti-RFP antibody. Tissue: DsRed transgenic mouse retina. Fixation: 4% PFA. Blocking: 3% BSA, 0.3% Triton Primary antibody: RFP-biotin antibody at 1:100 for 12 h at 4°C. Secondary antibody: Alexa488 secondary antibody at 1:10000 for 4 hours at RT. Localization: RFP is nuclear and occasionally cytoplasmic. Staining: labeled in red.
Western Blot of Chicken Anti-RFP Antibody Biotin Conjugated. Lane 1: Super Signal Molecular Weight. Lane 2: 50 ng of RFP. Primary Antibody: Chicken Anti-RFP biotin conjugated at 1:1000 overnight at 2-8°C. Secondary Antibody: HRP Streptavidin (p/n orb348767) 1:40000 for 30 mins at RT. Block: BlockOut Universal Blocking buffer (p/n orb348644). Expect: 27 kDa.
Mouse | |
31.25-2000 pg/mL | |
16.1 pg/mL |
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