Retinol Binding Protein 4 Antibody / RBP4

• Catalog Number: orb699768
• Category: Antibodies
• Description: Retinol (Vitamin A) is transported in the blood bound to its carrier protein, retinol-binding protein (RBP), also designated plasma retinol-binding protein (PRBP) or RBP4. A member of the lipocalin family, RBP conveys retinol from stores in the liver to peripheral tissues. In plasma, RBP binds transthyretin (TTR, formerly called prealbumin) to prevent glomerular filtration of low molecular weight RBP in the kidneys. The stability of this complex holds diagnostic importance because the molar ratio of RBP:TTR provides an indirect way to indicate marginal Vitamin A deficiency. Vitamin A deficiency blocks the secretion of RBP, resulting in defective delivery and supply to epidermal cells. Originally identified solely as a transporter protein, recent studies correlating increased levels of RBP expression in adipose tissue with Insulin resistance have generated research into the possible roles the protein may play in the pathogenesis of type 2 diabetes and obesity.
• Species/Host: Mouse
• Clonality: Monoclonal
• Clone Number: RBP4/4041
• Tested applications: IHC-P
• Reactivity: Human
• Isotype: Mouse IgG2b, kappa
• Immunogen: A portion of amino acids 29-148 from the human protein was used as the immunogen for the Retinol Binding Protein 4 antibody.
• Dilution range: Immunohistochemistry (FFPE): 1-2ug/ml for 30 minutes at RT
• Purity: Protein G affinity chromatography
• Conjugation: Unconjugated
• Formula: 0.2 mg/ml in 1X PBS with 0.1 mg/ml BSA (US sourced), 0.05% sodium azide
• Hazard Information: This Retinol Binding Protein 4 antibody is available for research use only.
• UniProt ID: P02753
• Storage: Store the Retinol Binding Protein 4 antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide).
• Buffer/Preservatives: 0.2 mg/ml in 1X PBS with 0.1 mg/ml rAlbumin (US sourced), 0.05% sodium azide
• Note: For research use only
• Application notes: Optimal dilution of the Retinol Binding Protein 4 antibody should be determined by the researcher.
• Expiration Date: 12 months from date of receipt.

IHC staining of FFPE human renal cell carcinoma with Retinol Binding Protein 4 antibody (clone RBP4/4041). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

IHC staining of FFPE human liver carcinoma with Retinol Binding Protein 4 antibody (clone RBP4/4041). HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.

Analysis of HuProt (TM) microarray containing more than 19000 full-length human proteins using Retinol Binding Protein 4 antibody. These results demonstrate the foremost specificity of the RBP4/4041 mAb. Z- and S- score: The Z-score represents the strength of a signal that an antibody (in combination with a fluorescently-tagged anti-IgG secondary Ab) produces when binding to a particular protein on the HuProt (TM) array. Z-scores are described in units of standard deviations (SD's) above the mean value of all signals generated on that array. If the targets on the HuProt (TM) are arranged in descending order of the Z-score, the S-score is the difference (also in units of SD's) between the Z-scores. The S-score therefore represents the relative target specificity of an Ab to its intended target.