Retinoblastoma K860 Me1 antibody

• Catalog Number: orb420377
• Category: Antibodies
• Description: Retinoblastoma K860 Me1 antibody
• Species/Host: Rabbit
• Clonality: Polyclonal
• Tested applications: DOT, ELISA, WB
• Reactivity: Human
• Isotype: IgG
• Immunogen: Anti-Retinoblastoma [Monomethyl Lys860] Antibody was prepared from whole rabbit serum produced by repeated immunizations with a methylated and acetylated synthetic peptide surrounding lysine 860 of human RB1 conjugated to Keyhole Limpet Hemocyanin (KLH).
• Concentration: 0.99 mg/ml
• Dilution range: ELISA: 1:10,000-1:50,000, WB: 1.0 ug/ml
• Form/Appearance: Liquid (sterile filtered)
• Purity: This affinity purified antibody is directed against human Retinoblastoma. This product was affinity purified from monospecific antiserum by immunoaffinity purification. This antibody reacts with human RB1 surrounding aa860 and is specific for K860 Me1. Blast analysis of the sequence of the immunogen shows 100% identity with human and 92% identity to rat and mouse.
• Conjugation: Unconjugated
• UniProt ID: P06400
• NCBI: NP_000312.2
• Storage: Store vial at -20° C prior to opening. Aliquot contents and freeze at -20° C or below for extended storage. Avoid cycles of freezing and thawing. Centrifuge product if not completely clear after standing at room temperature. This product is stable for several weeks at 4° C as an undiluted liquid. Dilute only prior to immediate use.
• Buffer/Preservatives: 0.01% (w/v) Sodium Azide
• Alternative names: Retinoblastoma-associated protein, p105-Rb, pRb, p
• Note: For research use only
• Application notes: Anti-Retinoblastoma K860 Me1 Antibody was tested for use in ELISA, Dot Blot, and Western Blot. Expect a band at approximately 106.1kDa in MOLT-4 and other appropriate cell lysates or tissues. Although not tested, expect reactivity in immunoprecipitation and immunohistochemistry.
• Expiration Date: 12 months from date of receipt.

Dot Blot of Rabbit Anti-Retinoblastoma [Monomethyl Lys860] Antibody. Lane 1: Unmodified peptide Retinoblastoma peptide. Lane 2: Retinoblastoma [Monomethyl Lys860] peptide. Load: 1.0, 0.5, and 0.25 picomoles of peptide. Primary antibody: Retinoblastoma [Monomethyl Lys860] antibody at 1.0, 0.5, and 0.25 µg/ml for o/n at 4°C. Secondary antibody: Gt-A-Rb IgG HRP Conjugated Secondary Antibody (p/n orb347654) at 1:40000 in BLOTTO Blocking Buffer 30 min at RT. Block: 5% BLOTTO (p/n orb348624) 30 min at RT.

Western Blot of Rabbit anti-Retinoblastoma [Monomethyl Lys860] antibody. Lane 1: MW ladder opal pre-stained. Lane 2: MOLT-4 WCL (p/n orb348696). Load: 35 ug per lane. Primary antibody: Retinoblastoma antibody at 1:1000 for overnight at 4°C. Secondary antibody: rabbit secondary HRP antibody (p/n orb347654) at 1:70000 for 30 min at RT. Block: BlockOut (p/n orb348644) 30 min at RT. Predicted/Observed size: expect 106 kDa for Retinoblastoma protein.