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Catalog Number | orb1527302 |
---|---|
Category | Antibodies |
Description | RelA, Phospho (S536) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | IHC, WB |
Reactivity | Human |
Immunogen | Surrounding serine 536 |
Concentration | 200 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended; IHC - 1:200 - 1:1,000. Epitope retrieval with Tris-EDTA pH 9.0 is recommended for FFPE tissue sections. |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RelA, Phospho (S536) |
UniProt ID | Q04206 |
NCBI | NP_068810.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-buffered Saline containing 0.1% rAlbumin and 0.09% Sodium Azide |
Alternative names | p65; NF-kappa-B transcription factor p65; v-rel re Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human phospho RelA-S536 by western blot. Samples: Whole cell lysate (15 and 50 µg) from Jurkat cells treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: Affinity purified rabbit anti-Phospho RelA (S536) antibody orb1527302 used for WB at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds. Lower panel: Western blot for total RelA using affinity purified rabbit anti-RelA antibody at 0.1 µg/ml with exposure time of 75 seconds.
Detection of human Phospho RelA (S536) by immunohistochemistry.Sample: FFPE section of human ovarian carcinoma. Antibody: Affinity purified rabbit anti-Phospho RelA (S536) (Cat. No. orb1527302) used at a dilution of 1: 200 (1µg/ml). Detection: DAB Counterstain: IHC Hematoxylin (blue).
IH, IP, WB | |
Human, Mouse, Porcine, Primate, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IHC-P, WB | |
Bovine, Canine, Equine, Gallus | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
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