You have no items in your shopping cart.
You have no items in your shopping cart.
Catalog Number | orb1527299 |
---|---|
Category | Antibodies |
Description | RelA, Phospho (S529) Antibody |
Species/Host | Rabbit |
Clonality | Polyclonal |
Tested applications | WB |
Reactivity | Human |
Immunogen | Surrounding serine 529 |
Concentration | 1000 µg/ml |
Dilution range | WB - 1:2,000 - 1:10,000; IP - Not recommended |
Form/Appearance | Whole IgG |
Conjugation | Unconjugated |
Target | RelA, Phospho (S529) |
UniProt ID | Q04206 |
NCBI | NP_068810.2 |
Storage | 2 - 8°C |
Buffer/Preservatives | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
Alternative names | p65; NF-kappa-B transcription factor p65; v-rel re Read more... |
Note | For research use only |
Application notes | Format: Whole IgG |
Expiration Date | 12 months from date of receipt. |
Detection of human Phospho RelA (S529) by western blot. Samples: Whole cell lysate (50 µg) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibody: Affinity purified rabbit anti-Phospho RelA (S529) antibody orb1527299 used at 0.1 µg/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.
Detection of human Phospho RelA (S529) by western blot of immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells that were treated with TNF alpha and Calyculin A (+) or mock treated (-). Antibodies: RelA was immunoprecipitated with affinity purified rabbit anti-RelA antibody. Immunoprecipitated RelA was treated with phosphatase or mock treated. Affinity purified rabbit anti-Phospho RelA (S529) antibody orb1527299 used for WB at 1 µg/ml.
IH, WB | |
Human, Primate | |
Rabbit | |
Polyclonal | |
Unconjugated |
IH, WB | |
Human, Mouse, Rat | |
Rabbit | |
Polyclonal | |
Unconjugated |
FC, IP, WB | |
Human | |
Rabbit | |
Monoclonal | |
Unconjugated |
Filter by Rating